Fig. 2. Expression pattern of tsh relative to hth and EY in eye and wing discs. tsh-GAL4 (Shiga et al., 1996) driven UAS-GFP and hth1422-4 (an enhancer trap insertion in hth) (Kurant et al., 1998) and anti-EY antibody (Halder et al., 1998) were used to examine the expression patterns of tsh, hth and EY (GFP, green; hth-lacZ, red; and EY, blue) in (a) first instar eye-antenna disc, (b) second instar eye disc, (c) early third instar eye disc, (d) late third instar eye disc and (e) late third instar wing disc. Differentiated photoreceptors in d were marked by ELAV (blue). (AN, antenna disc; E, eye disc)