Fig. 4. tsh mutant clones can cause ventral enlargement and dorsal suppression in eye. (a) tsh⁸ clones were generated by X-ray irradiation of w; tsh⁸/tshA8 larvae. The tsh⁸ clones were marked by the loss of the tshA8 mini-white reporter in the adult eye. (b) Wild-type expression of the tshA8 reporter, a P[lacW] insertion at tsh locus and expresses in the anterior half of the adult eye. (c,d) tsh⁸ clones located at the ventral margin caused overgrowth (arrow). (e-g) tsh⁸ clones were not marked in the disc, but eye discs treated for clone induction could have ventral enlargement (e,f, arrow) and ectopic eye field (g, arrow). (h,i) tsh⁸ clones located in the anterior dorsal eye can suppress eye (arrow). (j) tsh⁸ clone induction can cause nearly complete elimination of the dorsal eye field (arrow).