Fig. 7. DV differential functions of tsh in the antennal disc but not in the wing disc. (a-d) Effects of tsh misexpression in wing disc (anterior is towards the left and ventral is towards the top). (a) Act>tsh clone (GFP, green) could induce HTH (red) in a cell-autonomous manner. (b) Act>tsh located in the wing pouch region did not suppress WG (red). (c) dpp>tsh caused a splitting of the wing pouch by ectopic induction of HTH (red) along the AP compartmental boundary (arrow). (d) bi>tsh could split the wing pouch by ectopic induction of HTH (red). (e) bi>hth did not suppress WG (green) and did not split the wing pouch. (f-j) Effects of tsh misexpression in the antennal disc. Note that the DV axis in the antennal disc (dorsal is upwards and ventral is downwards in these figures) is inverted compared to that of the eye disc. (f,g) Act>tsh clone (GFP, green) located in the ventral wg expression domain (arrow) resulted in duplication of the antenna field in the disc (f) and in an adult (g). (h) Act>tsh clone (GFP: green) located in the anterior (arrow) or posterior (arrowhead) proximal regions did not affect HTH (red) and caused no ectopic eye induction (ELAV, blue). (i) Act>tsh clone in the DP region can cause overgrowth. (j) An Act>tsh clone in the anteroventroproximal region caused ectopic eye formation (ELAV, blue, arrow).