Fig. 6. Microfilaments are functional in oocytes prior to GVBD and cortical granule translocation. Shown are oocytes treated for 30 minutes with the given reagent and then 10 minutes with FM1-43 (top) and DIC (bottom). Images were recorded by confocal microscopy. The plasma membrane is intensely labeled in all cells because of exposed surface area. Endocytosis is inhibited reversibly in latrunculin A and cytochalasin B, respectively, but no inhibition is seen in cells treated to disrupt microtubules (C). Scale bar: 25 µm. The endocytic vesicles are approximately 0.5 µm in diameter.