Fig. 8. Cortical granules associate with microfilaments at GVBD. Oocytes having undergone GVBD in vitro were subjected to stratification either in the absence (A-C) or presence (D-F) of cytochalasin D. Cortical granules were then immunolabeled and their position quantified. (A,D) immunolabeled cortical granules; (B,E) Hoechst label; (C,F) DIC images. Control oocytes are as in previous figures, prior to GVBD. Meiotic oocytes have undergone GVBD, although the precise stage of meiosis is not known. (G) Quantitation of multiple experiments; asterisks indicate changes that are significantly different from control oocytes at P>0.01 (confidence level determined by Student’s t-test analysis). Scale bar: 25 µm.