Fig. 7. Amino acid alignment and phylogenetic tree of P4.1R family members. (A) Alignment of the conserved FERM domain in P4.1R proteins from zebrafish, human, mouse, Xenopus and Drosophila (Dm coracle). Shaded blocks indicate similar and identical amino acids. (B) A phylogenetic tree of the protein P4.1R amino acid sequences reveals an evolutionary conservation between members of P4.1R superfamily. The MegAlign application in DNAStar software was used for alignment and construction of the phylogenetic tree.