Fig. 5. Anteroposterior patterning in embryos derived from csn5p germline clones. Embryos, oriented with dorsal upwards and anterior towards the left, were hybridized with digoxigenin-labeled antisense RNA probes. (A,B) giant expression in wild-type (A) and mutant (B) embryo undergoing cellularization. In the mutant embryo, giant expression is tilted towards the anterior. (C,D) Cellularizing wild-type and mutant embryo after hybridization with a knirps probe. knirps expression is tilted towards the anterior in the mutant embryo. (E-G) even-skipped expression in wild-type and mutant embryos undergoing cellularization. The seven-stripe even-skipped pattern is altered to a variable extent in csn5p mutant embryos. The embryo in F has an expanded stripe 2 and diminished stripe 5, whereas the embryo in G is more severely affected. (H-J) Embryos undergoing germband elongation hybridized with an engrailed probe. The mutant embryos in I,J have failed to undergo the cell movements of gastrulation. The normal engrailed pattern includes 14 stripes that extend along the length of the germband. In mutant embryos, a striped engrailed pattern can no longer be observed.