Fig. 1. Expression of transgenic (TG) (A-C) and endogenous (D-F) Noggin in developing eyes. In situ hybridization using a ³⁵S-labeled SV40 riboprobe show that Noggin transgene was specifically expressed in the lens but at different levels in different families (A-C). Three representative families, OVE1198 (A), OVE1195 (B) and OVE1194 (C) are shown here. In wild-type (WT) developing eyes, endogenous Noggin transcripts were not detected in E13 eyes by in situ hybridization (data not shown). In E18 (D), P1 (E) and P7 (F) eyes, endogenous Noggin transcripts were present in most of the RPE except for the anterior region. The gaps between the neural retina (NR) and the RPE indicated by asterisks (D,F) are processing artifacts. Abbreviations: CE, ciliary epithelium; L, lens. Scale bars: 100 µm.