Fig. 5. LET-99 localization depends on PAR-3 and PAR-2. Confocal images of par-3 embryos (A-H) and par-2 embryos (I-P) stained with anti-LET-99 antibodies (top panels) and DAPI (bottom panels). (A,E,I,M) One-cell prophase embryos. (B,F,J,N) One-cell metaphase embryos. (C,G,K,O) One-cell late anaphase embryos. (D,H,L,P) Two-cell prophase embryos. Arrows indicate the boundaries of the LET-99 central band in par-3 and of the posterior domain in par-2 embryos. Large arrowheads indicate polar bodies. Small arrowheads in B,J and C,K indicate the metaphase plate and spindle midzone-associated staining of LET-99, respectively; the intensity of these two patterns varies in both wild type and par mutants. Scale bar: 10 µm.