Fig. 7. MUK protein associated with microtubules and Golgi apparatus. (A) Primary culture of E16 cortical cells was stained with anti--tubulin antibody (green) and anti-MUK antibody (red). Nuclei were labeled with DAPI (blue). (B) Higher magnification of a cell prepared as in A, showing the association of the dotted structure containing MUK with microtubules. Many MUK-containing dotted structures are located at the tip of microtubules (arrows). (C) Cells were stained with anti-MUK antibody (left panel, red) and anti-GM130 antibody to visualize Golgi apparatus (middle panel, green). Merged view with nuclear staining with DAPI (blue) is also shown (right panel). Note the certain fraction of MUK localized on Golgi apparatus (arrows). Scale bars: 10 µm.