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Fig. 2. Ectopic expression of DMyb causes malformation in the larval wing disc. Imaginal wing discs dissected from wandering third instar larvae, posterior to the right in all figures. (A-C) Fluorescent micrographs showing the expression patterns of the en-Gal4 and sd-Gal4 drivers: (A) en-Gal4/UAS-GFP, (B) en-Gal4/UAS-{Delta}DMyb and (C) sd-Gal4/UAS-GFP. Red, DAPI-staining to visualize nuclei; green, either (A,C) GFP fluorescence or (B) staining with an antibody raised against the DNA-binding domain of DMyb (Jackson et al., 2001). Note the difference in the shape of the disc when {Delta}DMyb is expressed in the posterior compartment. (D-F) Micrographs using differential interference contrast (DIC) optics show a comparison between the appearance of (D) a control en-Gal4/+ wing disc and discs in which DMyb has been ectopically expressed; (E) en-Gal4/UAS-DMyb; and (F) sd-Gal4/UAS-{Delta}DMyb. Scale bars: in A, 0.05 mm for A-C; in D, 0.05 mm in D-F.