Fig. 4. Defects in stet mutant ovarioles. DAPI staining of (A) wild-type and (B-D) stet mutant ovarioles in the same magnification, apical tips towards the left, germarium (g) at the apical tip. Scale bar: 0.1 mm. (A) Wild-type ovariole showing egg chambers at different developmental stages; nurse cells (nc) and oocyte (oc) as indicated, egg chambers contain 16 germ cells. (B) Ovariole from a young stet mutant female showing egg chambers at many stages. Note egg chambers with different sized nurse cell nuclei (arrowheads). (C) Egg chambers from a young stet mutant female showing abnormal numbers of germ cells, as indicated. (D) Ovariole from a 10-day-old stet mutant female showing germ cells at the apical tip (arrow) and degenerating egg chamber (arrowhead).