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Fig. 7. TUNEL analysis in E10.5 PO-Cre::R26R::Fgf8neo/– mutant demonstrates NCCs that are dying. Frontal section through first PA demonstrates ß-Gal-positive cells (A), TUNEL-positive cells (B) and overlap (C). Blow up of boxed area in C clearly demonstrates double labeled (lacZ and TUNEL positive) cells (D). Nt, neural tube; E, eye; BA1, pharyngeal arch 1.