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Fig. 5. Whole-mount in situ hybridization analysis of Sertoli cell marker gene expression in control and mutant E13.5 XY gonads. Left panels: Dhh (top), Sox9 (middle) and Mis (bottom). Right panels: Sox9 (top) and Mis (bottom). g, gonad; m, mesonephros. None of the Sertoli cell markers examined is expressed in mutant gonads.