Fig. 7. Expression of casanova rescues late differentiation of endoderm and early endodermal marker expression in oep and bon mutants. Compared with 24 hpf MZoep (A), Zoep (B) and bon (C) controls, which are deficient in endodermal structures and in the endodermal differentiation marker fkd7, casanova overexpression can sometimes restore the differentiation of pharynx in MZoep mutants (D) (11%, n=97) and efficiently restores differentiation of pharynx and gut in Zoep (E,F) (88%, n=42) and bon mutants (G) (100%, n=17), as evidenced by fkd7 expression (arrowheads). Overexpression of casanova also increases the number of cells expressing the early endodermal markers sox17 and foxa2 in MZoep (H-K) and bon (L-O) embryos.