Fig. 2. Migrating cells extend long leading processes towards the direction of the migration. Confocal images of whole mount explants injected with DiI at the rhombic lip. The pictures on the right illustrate the regions boxed on the diagrams. (A,B) Explant injected at E12.5. Close to the pial surface long leading processes, labeled with DiI, extended towards the midline (A). At a deeper level, many labeled axons have crossed the midline and turned anteriorly (B). (C,D) Explant injected at E12.5 and fixed after 1DIV. DiI-labeled cells emerged from the rhombic lip. These migrating cells have a unipolar shape with a long leading process. (E) Explant injected at E12.5 and fixed after 2DIV. The migrating cells have already reached the side contralateral to the injection. Scale bars: 50 µm.