Fig. 3. dap expression in the early central nervous system. Wild-type embryos (A-C) and prospero mutant embryos (D) were double labeled with antibodies against p27DAP (green) and Prospero (red), and two complete segments of the developing nervous system are shown. At stage 10, co-expression of p27DAP and Prospero is readily detected in the MP2 neuroblasts (arrowheads) but never in the first GMCs and neurons, which are labeled by anti-Prospero. A merged image is shown in A and the corresponding p27DAP labeling in B. p27DAP is also detected in MP2 of prospero mutant embryos (D). At stage 11, a merged image reveals co-expression of p27DAP and Prospero in additional cells of the central nervous system in wild-type embryos, as well as cells expressing either p27DAP or Prospero (C).