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Fig. 5. prd is required for accessory gland maturation. Expression of Acp26Aa protein (A,D,G), Gsb (B,E,F) and SP (C,F,I) in accessory glands of wild-type males (A-C), or Df(2L)Prl/prd2.45; prd-GsbN+PrdC/prd-GsbN+PrdC males carrying no (D-F) or one copy of a prd-mf9 transgene recombined onto the Df(2L)Prl chromosome (G-I) is detected by rabbit antisera against Acp26Aa and Gsb, or visualized by X-Gal staining of the product from an sp-lacZ reporter gene.