Fig. 7. Sequential generation of the MB neurons and their projections. (A,D) Neuroblast clone (green/white) induced in the first instar. (B,E) Two-cell clone induced in the first instar. (C,F) Neuroblasts clone induced at the beginning of the third instar. Clones are labeled with elev-GAL4 and UAS-mCD8::GFP. (A-C) Dorsal views of the Kenyon cells labeled with anti-EY (blue). Late third instar stage. Stars indicate the location of the four-MB neuroblasts. The position of the GFP marked neuroblast is labeled with a yellow star. (D-F) Lateral views showing the peduncle, dorsal and medial lobes. Insets in D-F are optical cross sections of the peduncle. The peduncle and lobes are labeled with anti-FAS II (magenta). Arrow in the inset indicates the core. DL, dorsal lobe; cx, calyx; ML, medial lobe; Ped, peduncle. Scale bars: in A, 40 µm in A-C; in D, 40 µm for D-F. Genotypes: GAL4c¹⁵⁵, hs-FLP/X or Y; G13, UAS-mCD8::GFP/G13, tubP-GAL80.