Fig. 7. The phenotype of Sox9-depleted embryos can be rescued by restoring Sox9 expression. (A) Rescue experiments were performed by injection of an animal dorsal blastomere at the eight-cell stage. (B) Representative case of Slug whole-mount in situ hybridization of stage 17 embryos injected with 100 pg of Sox9 plasmid (Sox9) or 5 ng of Sox9-AS (Sox9-AS), or a combination of both (Sox9-AS+Sox9) or a combination of Sox9-AS and 100 pg of a control GFP plasmid (Sox9-AS+GFP). RNA encoding the lineage tracer ß-galactosidase was co-injected to identify the injected side (red staining), right side in all cases. Dorsal view, anterior is at the top. (C) Quantification of the in situ hybridization results. n, number of cases analyzed.