Fig. 3. Defects in pop-1(Sys) hermaphrodite gonad. (A-C) Animals carrying lag-2::GFP. Animals in A and C carry qIs56[lag-2::GFP], animal in B carries qIs19[lag-2::GFP]. Both transgenes have similar GFP expression in the DTC, but qIs56 has brighter GFP expression in the ventral nerve cord than qIs19. (A) Wild-type L3, SPh and one gonadal arm are shown. The DTC caps the end of the elongating arm and expresses lag-2::GFP brightly (arrow). Somatic gonadal cells within the SPh (black arrowheads) cluster centrally. (B,C) pop-1(q645) and pop-1(RNAi) animals have no gonadal elongation and no bright lag-2::GFP expression. Somatic gonadal cells are arranged around the gonadal periphery (black arrowheads). (D-F) Animals expressing cdh-3::GFP. (D) Wild-type L3. The AC marker cdh-3::GFP is expressed in a single cell (white arrowhead). (E,F) pop-1(q645) and pop-1(RNAi) L3 animals can have multiple ACs (white arrowheads).