Fig. 4. Expression of BrdU and TuJ1 in wild-type and Pax6^Sey/Sey E12.5 cortex. Parasagittal sections through cortex of (A) wild-type and (B) Pax6^Sey/Sey E12.5 telencephalon. Dams were injected at 0 and 2 hours and embryos collected at 2.5 hours after initial injection. Broken line indicates boundary of VZ. There is a clear increase in double-labelled cells (BrdU, brown; TuJ1, purple) in the mutant (B) compared with the wild-type (A) littermate. Scale bar: 60 µm.