Fig. 6. Immunocytochemical analysis of dissociated cortical cells in culture. (A,C,E,G,I) Wild-type and (B,D,F,H,J) Pax6^Sey/Sey dissociated cortical cells after 12.75 hours (E-J) and 24 hours (A-D) in culture. (A-D) BrdU labelling in cultures 24 hours after plating (11.25 hours after the end of the BrdU pulse). (A,B) Double immunostaining for BrdU (brown) and MAP2 (blue). In contrast to the wild-type BrdU-labelled cells, Pax6^Sey/Sey-labelled cells were mainly in large clusters. (C,D) Many mutant BrdU-labelled cells were grouped as to suggest cell-chain migration. Inset in D shows a panoramic view of mutant cortical cell-chain formation in culture. (E-J) Immunodetection of (E,F) neuronal antigen class III ß tubulin (TuJ1), (G,H) nestin and (I,J) MAP2 (blue) and RC2 (brown). Arrowheads in I,J show cells expressing both markers. Scale bars: 24 µm in A,B,E-J; 16 µm in C,D.