Fig. 8. Overexpression of HSV-GFPBrn3c in retinal explants. (A) HSV-GFP-Brn3c amplicon. The coding region of Brn3c was inserted between the XbaI and BamHI sites of the amplicon vector. GFP was driven by the CMV promoter, and Brn3c by the HSV IE4/5 promoter. The vector without the Brn3b insert served as a control. (B) Low-magnification view of a Brn3b^–/– retinal explant infected with the HSV-GFP control amplicon. (C) An enlarged view of the peripheral region of the same sample as in B, showing a small number of abnormal neurites emanating from the tissue body. (D) Low-magnification view of a Brn3b^–/– retinal explant infected with the HSV-GFP-Brn3c amplicon. (E) An enlarged view of the peripheral region of the same sample as in D showing a large number of neurites emanating from the tissue body. Inset shows branched structures (arrows).