Fig. 5. Decreased activating histone modifications on one hermaphrodite chromosome pair. (A-E) PD7271 (A1-A3) or N2 (B-G) hermaphrodite ovaries were fixed with paraformaldehyde and stained with antibodies recognizing histone H3 methyl-K4 (A), H3 acetyl-K9, –14 (B), H3 phospho-S10 (C), histone H4 acetyl-K8 (D) and H4 acetyl-K16 (E). In A-E, antibody staining is false-colored green and DAPI counterstain is false-colored red. Arrowheads in A1-A3 point to transgenes identified as in Fig. 2. Arrows in A3-E point to the under-labeled chromosome; arrowhead in C points out a concentration of the H3 phospho-S10 modification that is otherwise absent from this chromosome. (F,G) N2 hermaphrodite germ cells were simultaneously stained with -H3 dimethyl-K4 (F; green) and mAb H5 (G; green), and counterstained with DAPI (F,G; red). Arrowheads indicate chromosomes under-stained by both antibodies. (H,I) -H3 dimethyl-K4 staining of pachytene nuclei in strain carrying autosomal duplication sDp1 (H) and the activated transgene (KW1336; I). The free duplication and the transgene are circled in H,I, respectively.