Fig. 6. The under-modified chromosome is the X chromosome. (A) Hermaphrodite animals homozygous for an X:autosome reciprocal translocation [mnT10(X:V)] were stained with -H3 dimethyl-K4 (green) and counterstained with DAPI (red). Arrows point to chromosomes in a nucleus that are each half-labeled by the antibody. Scale bar: 5 µm. (B) Hermaphrodite germ cells were probed simultaneously by antibody -H4 acetyl-K12; (‘AcHis4’) and by fluorescent in situ hybridization (FISH) using probes to both ends of the X chromosome. Scale bar: 5 µm in A.