Fig. 2. Rab11 expression in wild-type oocytes and in rab11^P2148 GLCs. (A-E) Confocal immunolocalization of Rab11 and Osk. (A-C) wild-type oocytes. (A) Stage 7 oocyte showing strong accumulation of Rab11 along the lateral and posterior cortex, and in a perinuclear compartment (arrowhead). In these and in all subsequent panels, anterior is towards the left. (B) Stage 10 oocyte showing specific accumulation of Rab11 at the posterior pole. (C) Early stage 10 oocyte doubly labeled for Rab11 (red) and Osk (green). The two proteins show near perfect colocalization at the posterior pole as evident by the mixed (yellow) fluorescent signal. (D-E) Confocal immunolocalization of Rab11 in rab11^P2148 GLCs. (D) Late stage 7 oocyte showing normal accumulation of Rab11 in perinuclear compartment and along the cell cortex. (E) Early stage 10 oocyte showing diffuse accumulation of Rab11 throughout ooplasm with slight enrichment along cell cortex. (F) Rab11-GFP expression in a living wild-type stage 10 oocyte.