Fig. 3. Transferrin recycling in wild-type oocyte and in rab11^P2148 GLCs. (A,B) A wild-type stage 9 oocyte cultured with Texas Red-transferrin for one minute followed by a 20 minute wash in media alone. (A) Red channel showing the accumulation of transferrin in the perivitelline compartment and in vesicles at posterior pole of the oocyte. (B) Merged image showing transferrin (red) and Rab11-GFP (green). Inset shows an enlarged view of posterior pole, where the accumulation of transferrin in intracellular vesicles is clearly evident. (C) rab11^P2148 GLC cultured with transferrin as described above. Most of the transferrin is in the ooplasm, not in the perivitelline compartment. (D-G) FITC-Lectin labeling of plasma and vitelline membranes. (D-E) Plasma membrane labeling with Lycopersicon esculentum lectin. (F-G) Vitelline membrane labeling with Datura stramonium lectin. (D,F) Wild type. (E,G) rab11^P2148 GLCs.