Fig. 4. Rab11-dependent localization of -adaptin to the posterior pole of the oocyte. (A-C) Wild-type stage 9 oocyte doubly labeled for Rab11 (red) and -adaptin (green). (C) Merged image. (D) Merged image of a stage 10 oocyte doubly labeled for Rab11 (red) and -adaptin (green). In both oocytes, -adaptin is abundant all along the plasma membrane and in a posterior compartment that is close to, but clearly distinct from, the Rab11 expression domain. (E-G) A late stage9/early stage 10 rab11^P2148 GLC doubly labeled for Rab11 (red) and -adaptin (green), where the merged image is shown in G. Neither protein is found at high levels at the posterior pole of the oocyte.