Fig. 3. EKLF and GATA1 gene expression in the presence of selected cytokines. Gene expression was monitored (by semi-quantitative RT/PCR) in EBs differentiated for 8 days in SR1 in the absence (–) or in the presence (+) of a cytokine cocktail that included BMP4, SCF, VEGF, T3 and erythropoietin. Pooled EBs from a single dish are shown in each lane.