Fig. 3. The ix region defined by RFLP mapping and P-element-mediated germline transformation. (A) 65 kb ix region. cDNAs and known genes are indicated below the phage and cosmid clones in the ix region, and the genomic rescue constructs 2GB and 1GS are shown below the cDNAs. (B) Restriction-site map of the ix region defined by the 2GB genomic rescue construct. Transcripts included in the 2GB construct are indicated as arrows. The extents of germline transformation constructs are shown below the map, with rescue results indicated. Triangles indicate the positions of inserted stop codons. B, BamHI; P, PstI; R, EcoRI; S, SalI.