Fig. 3. Expression of teneurin 2 constructs. (A) Models of the four teneurin 2 proteins encoded by the constructs used for transfection. (B) Immunoblots with anti-teneurin 2 of cell extracts of COS-7 cells transfected with constructs CTEY, TEY, CTE and TE as indicated. Lanes TEY1 and TE1 represent immunoblots with anti-teneurin 2 of cell extracts of stable cell clones of HT1080 cells selected after transfection with TEY or TE, respectively. (C) Immunostaining of COS-7 cells with anti-teneurin 2 after transfection with the constructs indicated reveals cell surface expression. Note the numerous filopodia of cells transfected with the constructs containing the cytoplasmic domains (CTEY and CTE).