Fig. 3. Phox2b and TH immunoreactivities in adrenal glands of E14.5 Mash1^–/– (B) and Mash1^+/+ (A) mice. Double immunofluorescence-staining using antibodies against TH (red cytoplasmic stain) and Phox2b (green nuclear stain). (A) In adrenal glands of wild-type mice virtually all Phox2b-immunoreactive cells are also positive for TH. (B) By contrast, adrenal glands of Mash1^–/– mice display many Phox2b-positive cells that are TH-negative (arrowheads). (C) Quantification of numbers of Phox2b and TH positive cells in the adrenal glands of wild-type and Mash1^–/– mice. Data are presented as mean±s.e.m. At least six adrenal glands of three different animals were analyzed per group. Scale bar: 50 µm.