Fig. 5. Co-expression of Lmo4 and LIM homeodomain proteins by neurons at earlier developmental stages. Localization of Lmo4 (red) and the LIM homeodomain proteins Isl1/Isl2 and Lim1/Lim2 (green) was determined by double-label immunohistochemistry in DRG sensory neurons (A,B) and in lumbosacral spinal motoneurons (C). At stage 26, Lmo4 is co-expressed with Isl1/Isl2 in DRG neurons (A). At stage 28, Lmo4 is excluded from DM cutaneous sensory neurons (B, dotted outline indicates the major region of non-overlap of Lmo4 and Isl1/Isl2). (C) At stage 26, Lmo4 is expressed in a subset of Lim1/Lim2-positive (1) LMC motoneurons at LS2 (arrows). The dorsomedial region of DRGs is located in the upper right in A,B. Lateral is towards the right in C. Scale bars: 25 µm in A,C; 50 µm in B.