Fig. 1. MEC-8 expression pattern. (A,C) Early embryos with anti-MEC-8 staining in all nuclei. (B) DAPI staining of the embryo shown in A. (D,E) Older embryos with anti-MEC-8 staining in hypodermal nuclei. (F) A lower focal plane of the same embryo as in E. This embryo was transgenic for _pPD37.48, an hlh-1::lacZ fusion construct (Krause et al., 1994); muscle cells stained with anti-ß-galactosidase and not with anti-MEC-8. (G) MEC-8::GFP in a 1.5-fold embryo. (H) Anti-LIN-26 hypodermal staining of the embryo shown in G, showing overlapping staining with anti-MEC-8. (I) A mec-8(u391) embryo lacking anti-MEC-8 nuclear stain. (J) The mid-body region of an L1 larva in which hyp7 nuclei were stained with anti-MEC-8. (K) DAPI stain of the larva shown in J. (L) The mid-body region of an adult stained with anti-MEC-8. The white arrow indicates ALML (touch neuron), and the black arrow indicates the vulva. Scale bar: 10 µm.