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Fig. 3. HrT regulates tbx5. (B,D,F,H) Embryos injected with 1.5 ng hrTMO(1) are compared with uninjected (A,C,E,G) embryos. (A-D) Dorsal views with anterior towards the bottom. (A,B) Expression of tbx5 at the 15-somite stage. (C,D) Expression of tbx5 at 24 hpf in the heart field. (E,F) Frontal views with anterior towards the top shows the expression of tbx5 at 33 hpf in the heart field. Note increased expression of tbx5 in hrTMO(1)-injected embryos (F). (G,H) Dorsal views with anterior to the top shows the expression of tbx5 at 33 hpf in the fin buds. (I-L) Embryos were injected with 0.2 ug of hrT-GR RNA and half were treated with dexamethasone (Dex) as indicated. (I,J) Frontal views with anterior to the top of 30 hpf embryos showing tbx5 expression in the heart. Note decreased tbx5 expression after GR-hrT induction. (K,L) Dorsal views with anterior towards the top of 30 hpf embryos, showing the expression of tbx5 in the developing fin buds. GR-hrT induction did not change the fin bud expression of tbx5.