(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Polar cells are required to specify border cells. fng mosaic follicles stained with Hoechst (DNA, white) and antibodies recognizing {pi}-MYC (fng+ cells, green), ß-galactosidase (slbo-lacZ, border cells, red) and FasIII (polar cells, cyan). In all figures, panels marked ' or '' show separate channels of the same follicle. (A) Stage 9 follicle with no fng+ anterior cells. No anterior polar cells are formed and no border cells are specified. Arrowhead indicates posterior polar cells. (B) Stage 10A follicle with only the two polar cells fng+ at the anterior. The polar and outer border cells are specified and properly migrate, even though all outer border cells are fng- (insets).