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Fig. 3. Polar cells are required to specify stretched cells. (A,B) fng mosaic follicles stained with Hoechst (DNA, white) and antibodies recognizing {pi}-MYC (fng+ cells, green), ß-galactosidase (MA33, stretched cells, red) and FasIII (polar cells, cyan). (A) Stage 9 follicle with no fng+ anterior cells. No anterior polar cells form and no stretched cells are specified. Arrowhead indicates posterior polar cells. (B) Stage 10B follicle with only a few fng+ cells at the anterior, including the polar cells (inset). The polar and stretched cells are specified properly, even though almost all stretched cells are fng-.