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Fig. 11. Effect of the ectopic expression of {Delta}HpEts on the expression of HpTb and gastrulation. (A) RT-PCR analysis of HpTb in embryos injected with {Delta}HpEts (+) mRNA and control (-) embryos. Total RNA was isolated from 50 control and 50 mRNA-injected embryos at 28 hours after fertilization. Southern blotting of RT-PCR products. (B) Control embryo and (C) embryo injected with {Delta}HpEts-mRNA at 48 hours after fertilization. Scale bar: 50 µm.