Fig. 4. HpTb protein is accumulated into PMC nucleus after blastula stage. (A) Developmental western blot. Thirty micrograms of protein prepared from embryos at various developmental stages were run in parallel. Detection procedure of HpTb protein is described in text. U, unfertilized egg; 16, 16-cell stage; M, morula; UB, unhatched blastula; HB, hatched blastula; MB, mesenchyme blastula; G, gastrula; Pr, prism; Pl, pluteus. (B,C) Immunostaining of embryos with antibodies to HpTb. (B) Morula; (C) mesenchyme blastula. Scale bar: 50 µm.