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Fig. 6. RT-PCR analysis of various tissue specific genes in control and embryos injected with HpTb morpholino. Total RNA was isolated from 50 control embryos and 50 embryos injected with HpTb morpholino antisense oligonucleotides at 28 hours after fertilization. Southern blotting of RT-PCR products. (-) and (+) indicate uninjected and injected embryos. The genes analysed are discussed in the text.