Fig. 9. Loss of organizing activity of micromeres by injecting with HpTb
morpholino. (A,B) Chimera composed of animal cap mesomeres from normal embryo
with the micromere quartet isolated from a 16-cell stage embryo developed from
zygotes injected with 7x108 molecules of HpTb
morpholino (A, vegetal view; B, lateral view). (C) Control embryo. (D,E) The
micromeres were labelled with rhodamine-dextran. Chimera composed of micromere
quartet from normal embryo with the animal cap mesomeres isolated from a
16-cell stage embryo developed from zygotes injected with
7x108 molecules of HpTb morpholino (D, lateral view;
E, vegetal view). The animal cap mesomeres were labelled with
rhodamine-dextran. Left, bright-field views; right, epifluorescence views.
Scale bar: 50 µm.