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Fig. 9. Xror2 can associate with Xwnt11, Xwnt5a and Xwnt8. Proteins were extracted from embryos injected with mRNA as indicated, immunoprecipitated (IP) with anti-FLAG antibody, and subjected to western blotting (WB) using anti-Myc antibody (top). The equivalent amounts of proteins generated from injected mRNAs were confirmed by western blotting of lysates using anti-Myc (middle) or anti-FLAG (bottom) antibody. White arrowheads, Wnt proteins. Amounts of mRNA (ng/embryo): globin, 1 (with Xror2KR-FLAG) or 2; Xror2KR-FLAG, 2; Exfz7-FLAG, 0.5; Xwnt11-Myc, 0.5 (with Exfz7-FLAG) or 1; Xwnt5a-Myc, 1; Xwnt8-Myc, 1.