Fig. 4. Ptch and Bmp4 expression in mutant kidneys. (A-H) In situ hybridization of Ptch and Bmp4 probes to E14.5 kidney (A,B,E,F) and ureter (C,D,G,H). The insets show Bmp4 expression in glomeruli. Scale bars: 100 µm in A,B,E,F; 50 µm in C,D,G,H. (I) RT-PCR of Bmp4 transcripts from E12.5 mesenchymal cells dissected from the ureter (lane 1) and those cultured without (lane 2) and with (lanes 3 and 4) the addition of N-SHH protein at concentrations indicated. PCR with ß-actin primers (ß-actin) indicated equal cDNA inputs in all lanes.