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Fig. 7. Smooth muscle differentiation in the wild-type (A,C,E,G) and Shh mutant (B,D,F,H) kidneys. Immunohistochemical staining for smooth muscle {alpha}-actin (brown) in the cross-sections of the proximal (A,B,E,F) and distal (C,D,G,H) ureters at the stages indicated. NB, newborn. Scale bar: 50 µm in A-D; 100 µm in E-H.