Fig. 3. Wg signalling regulates the expression of Eyes absent. axin^- M⁺ clones were induced 60±12 (A-H) and 108±12 hours AEL (I-K). In all panels, Eyes absent protein is shown in green and mutant clones are indicated by absence of red anti-lacZ staining. (A,B) In axin mutant clones (surrounded by broken lines) the expression of Eyes absent is abolished in the region anterior to the morphogenetic furrow (approximate furrow positions are marked with arrows in B and I); note, however, that Eyes absent is present in cells in the posterior region of the eye discs (arrowhead). Eyes absent expression is also eliminated in small internal clones anterior to the furrow (arrow in A). (C-E) A transverse section in the anterior region of the disc shown in A (red line) confirms that Eyes absent expression is autonomously eliminated from the clone. (F-G) In a more posterior transverse section from the same disc (blue line), we observe that Eyes absent is expressed in mutant cells. The overgrowth of the mutant cell leads to the folded morphology of the tissue. (I-K) Small clones induced late in eye development also prevent the expression of Eyes absent. However, in some mutant cells close to the morphogenetic furrow (position indicated with arrows in I). Eyes absent is still present at low levels (arrow in J). Panels J and K show a different disc from I, at higher magnification.