Fig. 5. axin^- clones induced in a non-Minute background also cause overgrowth and loss of eye specification genes. In this case, mutant clones are positively marked in by GFP staining (in red, for consistency with other figures), using the Gal4/Gal80 system. (A) Eyes absent expression (green) is abolished in anterior but not posterior axin^- clones. Red box: transverse section of a small anterior clone indicated by red line; mutant cells have grown out of the plane of the epithelium and do not express Eyes absent. Blue box: transverse section of a posterior clone indicated by blue line; mutant cells show extensive overgrowth (arrow) and some express Eyes absent. (B,C) Dachshund expression (green) is autonomously abolished in anterior axin^- clones (e.g. arrowhead in C). (D,E) Sine oculis expression is autonomously abolished in anterior axin^- clones (e.g. arrowhead in E). (D,E) Projections of several confocal sections; to demonstrate the autonomy of this loss, the inset in E shows a single confocal section of the clones indicated by a box in D (the central cells in the clone are not seen as they have grown out of the plane of the section).