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Fig. 4. (A-D) Distribution of phospho-histone 3 antigen in embryos injected with CsDelta1, CsDelta2 and CsNotch dsRNA. Confocal micrographs of flat preparations of opisthosomal segments of 160-hour embryos; anterior is towards the top. (A) The anti-phospho-histone 3 antibody stains single mitotic cells (arrow; green) that are uniformly distributed over the whole ventral neuroectoderm throughout neurogenesis in embryos injected with GFP dsRNA as a control. (B-D) The overall mitotic pattern is unchanged after injection of CsDelta1, CsDelta2 and CsNotch, respectively (arrows). Scale bar: 50 µm.