Fig. 5. Analysis of the of the expression of the flowering time genes FLC, FT, SOC1 and CO in wild-type and esd4 mutant plants. (A) Northern blot comparing FLC mRNA levels in 7-day-old Ler, esd4, fca-1, esd4 fca-1, fve-1, and esd4 fve-1 plants. The panel on the right is a longer exposure of the Ler and esd4 samples. (B) Northern blot comparing FT mRNA levels in 7-day-old Ler, esd4, fca-1 and esd4 fca-1 plants. The graph shows the mean level of FT gene expression relative to the UBQ10 control. In all graphs, black triangles represent Ler, black squares esd4, white triangles fca-1 and white squares esd4 fca-1; error bars indicate the standard error of the mean. (C) Northern blot comparing SOC1 mRNA levels in 7-day-old Ler, esd4, fca-1 and esd4 fca-1 plants. The graph shows the mean level of SOC1 gene expression relative to the UBQ10 control. Error bars indicate the standard error of the mean. (D) RT-PCR analysis of CO expression in 7-day-old wild-type and esd4 plants. The graph shows the level of CO gene expression relative to the UBQ10 control. Error bars indicate the standard error of the mean. (A-D) All experiments were performed three times using RNA from independently grown plant material.