Fig. 7. Western blot with anti-visinin mAb showing a 24 kDa visinin protein in E5.5, 6.5, 7.5 and 8.5 chick retinal extracts (A, lanes 1 to 4). The photoreceptors in the ONL of E7 (B) and E18 (C) retina are stained with visinin mAb. The examples of FACS analyses of RCAS- (D) and EnRaxLHD- (E) infected retina are shown, with the upper-right quadrants representing the visinin and p27 (virus infected) double-positive population; and the upper- and lower-right two quadrants representing all viral-infected cells (p27 positive). The average percentages of double-positive cells among all viral infected population are indicated in the F. n indicates the retina sample number. P values are based on the Student's t-test of two-tailed distribution as follows: ^*, P<0.005; ^**, P=0.152; ^***, P=0.226. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; RPE, retinal pigment epithelium.